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KMID : 1024420140180040427
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Food Engineering Progress
2014 Volume.18 No. 4 p.427 ~ p.433
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Purification of β-Mannanase from Bacillus sp. and the Effects of Picea abies Galactosyl Glucomannomannan Hydrolysates on the Growth Activity of Bifidobacterium spp.
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Lee Myung-Seok
Park Young-Seo
Park Gwi-Gun
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Abstract
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β-Mannanase from Bacillus sp. was purified by DEAE sephadex column chromatography. The specific activity ofthe purified enzyme was 21.57 units/mg protein, representing an 95.33-fold purification of the original crude extract.The final enzyme preparation obtained showed a single band on the SDS-PAGE and its molecular weight was esti-mated to be 38.9 kDa. Galactosyl glucomannan from Picea abies was hydrolyzed using the purified β-mannanase,and then the hydrolysates were separated by activated carbon column chromatography. The main hydrolysates werecomposed of galactosyl glucomannooligosaccharides with a degree of polymerization (DP) of 8 and 10. To investi-gate the effects of Picea abies galactosyl glucomannanooligosaccharides on the growth of the following: Bifidobac-terium longum, B. bifidum, B. animalis, B. breve, B. infantis, B. adolescentis, and B. auglutum, each Bifidobacteriumspp. was cultivated into the modified-MRS medium containing galactosyl glucomannooligosaccharides with DP 8 or10 as a carbon source. The growth of B. animalis increased 19.5-fold and 18.7-fold by the treatment of galactosylglucomannooligosaccharides with DP 8 and 10, respectively, compared to that of the standard MRS medium. B. bifi-dum grew 15.3 and 14.3-fold and B. longum grew 15.2 and 13.9-fold by the treatment of galactosyl glucomannoo-ligosaccharides with DP 8 and 10, respectively. Especially, galactosyl glucomannooligosaccharides with DP 8 wasmore effective than that with DP 10 on the growth of B. animalis, B. bifidum and B. longum.
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KEYWORD
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galactosyl glucomannooligosaccharides, Bacillus sp., β-mannanase, Bifidobacterium spp
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